Tubercle bacilli
staining is very difficult due to presence of fatty acid and alcoholic
material . Special type of staining is needed for the detection of tubercle
bacilli like Ziehl-Neelsen technique . Due to presence of higher group of
alcohol “Mykol” surrounding the body of the tubercle bacilli , the organism when stained is resistant to
the decolourising action of acdids and alcohol . for this property is called acid fast organism .
Properties
i)
Mycobacterium tuberculosis is gram positive , both acid
and alcoholic fast bacillus .
ii)
Bovine type takes uniform stain .
iii)
Human type takes granular stain with thinner , longer
and curved appearance .
iv)
After Ziehl – neelsen staining , when it is examined
under microscope , the bacillus appears like red rod and the tissue are blue .
v)
After staining with Auremin , a special stain ,
fluorescence appearance is shown by the organism in ultraviolet red .
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